Probing the Biology of Giardia intestinalis Mitosomes UsingIn VivoEnzymatic Tagging

Fermentation enzymes of Giardia intestinalis, pyruvate:ferredoxin oxidoreductase and hydrogenase, do not localize to its mitosomes.

It is becoming increasingly clear that the so-called remnant organelles of microaerophilic unicellular eukaryotes, hydrogenosomes and mitosomes, are significantly reduced versions of mitochondria. They normally lack most of the classic mitochondrial attributes, such as an electron transport chain and a genome. While hydrogenosomes generate energy by substrate-level phosphorylation along a hydro...

Rapid tagging and integration of genes in Giardia intestinalis.

We developed a series of plasmids that allow C-terminal tagging of any gene in its endogenous locus in Giardia intestinalis, with different epitope tags (triple hemagglutinin [3HA] and triple Myc [3Myc]) and selection markers (puromycin, neomycin, and a newly developed marker, blasticidin). Using these vectors, cyclin B and aurora kinase were tagged, expressed, and localized.

Fermentation enzymes of Giardia intestinalis , pyruvate : ferredoxin 3 oxidoreductase and hydrogenase , do not localize to its mitosomes

The Possible Pathogenicity of Giardia Intestinalis

Assistant Surgeon, Medical College Hospital, Calcutta, ortnerly Clinical Pathologist, Protozoology Depart-' ment, Calcutta School of Tropical Medicine.) The; role which Giardia inrcsnnuus may 'n the production of intestinal symptoms is still a disputed point. Nothing very definite can be stated one way or the other. It is claimed on the one hand' that it is frequently present in healthy persons...

The membrane potential of Giardia intestinalis.

Giardia intestinalis is a primitive microaerophilic protozoan parasite which colonises the upper intestine of humans. Despite the evolutionary and medical significance of this organism, its physiology is very poorly understood. In this study we have used a novel flow cytometric technique to make quantitative measurements of the electrical potential across the plasma membrane of G. intestinalis ...